Abstract
We have overexpressed the type II restriction endonuclease PvuII (R-PvuII) in E. coli, prepared large amounts of the homogeneous enzyme, and crystallized it with an oligonucleotide carrying a PvuII recognition site. The cocrystals are orthorhombic space group P2(1)2(1)2(1) with cell constants a = 95.8 A, b = 86.3 A, c = 48.5 A, and diffract X-rays to at least 2.7 A. There is a complex of two protein subunits and one oligonucleotide duplex in the asymmetric unit.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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Crystallization
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Crystallography, X-Ray*
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Deoxyribonucleases, Type II Site-Specific / chemistry*
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Deoxyribonucleases, Type II Site-Specific / isolation & purification
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Escherichia coli
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Gene Expression
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Molecular Sequence Data
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Oligodeoxyribonucleotides / metabolism*
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
Substances
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Oligodeoxyribonucleotides
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Recombinant Fusion Proteins
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CAGCTG-specific type II deoxyribonucleases
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Deoxyribonucleases, Type II Site-Specific