Abstract
A regulatable binary expression system for eukaryotes was recently developed based on the tetracycline repressor and its operator. Here we show that this system can be successfully applied to express antisense RNA and completely inhibit gene expression in a tetracycline-repressible fashion.
MeSH terms
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DNA, Complementary
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Gene Expression / genetics*
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HeLa Cells
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Humans
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RNA, Antisense / genetics
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Receptors, Interleukin / genetics
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Receptors, Interleukin-8A
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Repressor Proteins / metabolism*
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Tetracycline
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Transcription Factors / metabolism
Substances
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DNA, Complementary
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RNA, Antisense
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Receptors, Interleukin
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Receptors, Interleukin-8A
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Repressor Proteins
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Transcription Factors
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tetracycline resistance-encoding transposon repressor protein
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Tetracycline