[Isolation of intracellular inhibitors of bacterial RNAses on a column with immobilized Bacillus intermedius RNAse]

G E Bannikova; V P Varlamov

[Isolation of intracellular inhibitors of bacterial RNAses on a column with immobilized Bacillus intermedius RNAse]

Prikl Biokhim Mikrobiol. 1994 Mar-Apr;30(3):379-83.

[Article in Russian]

Authors

G E Bannikova, V P Varlamov

PMID: 8047536

Abstract

Intracellular inhibitors of RNases from Bacillus amyloliquefaciens and Bac. intermedius were isolated using affinity chromatography on covalently immobilized RNase from Bac. intermedius. The inhibitor of RNase from Bac. amyloliquefaciens was isolated from cells of E. coli HB 101 and purified to homogeneity. Proteins with molecular weights of 70, 36 and 20 kD possessing an inhibitory activity were found in the extract obtained from frozen cells of Bac. intermedius.

MeSH terms

Bacillus / enzymology*
Bacterial Proteins / isolation & purification*
Bacterial Proteins / pharmacology
Chromatography, Affinity
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Enzymes, Immobilized*
Molecular Weight
Ribonucleases / antagonists & inhibitors*
Ribonucleases / metabolism

Substances

Bacterial Proteins
Enzymes, Immobilized
barstar protein, Bacillus amyloliquefaciens
Ribonucleases