Abstract
The barley chloroplast psbA gene encoding D1 protein, one of the main photosystem II components, has been over-expressed in E. coli cells. The existance of two in vivo expression products, a protein with M(r) about 33.5 kDa, corresponding to the full-length precursor of the 32 kDa D1 mature form, and a truncated 29 kDa polypeptide was revealed. A modified D1 protein containing six histidine residues at the carboxy-terminus was also obtained. After isolation and renaturation, the ability of the recombinant D1 protein to bind atrazine and pigments from barley thylakoids was demonstrated.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism
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Base Sequence
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Blotting, Western
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Chromatography, Affinity
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Cloning, Molecular
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DNA
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics*
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Hordeum / genetics*
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Molecular Sequence Data
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Photosynthetic Reaction Center Complex Proteins / genetics*
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Photosynthetic Reaction Center Complex Proteins / metabolism
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Photosystem II Protein Complex
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Plant Proteins / genetics*
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Plant Proteins / isolation & purification
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Plant Proteins / metabolism
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Plasmids
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Bacterial Proteins
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Photosynthetic Reaction Center Complex Proteins
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Photosystem II Protein Complex
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Plant Proteins
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Recombinant Proteins
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DNA