Transformation of yeast cells by electroporation in combination with a screening method that avoids catabolic repression

Biotechniques. 1994 Apr;16(4):610-2.

Authors

J J Estruch¹, A Goff, L Crossland

Affiliation

¹ CIBA-GEIGY, Research Triangle Park, NC.

PMID: 8024779

No abstract available

MeSH terms

Binding Sites
Culture Media
DNA / metabolism
DNA-Binding Proteins
Electroporation*
Fungal Proteins / genetics
Gene Transfer Techniques*
Osmolar Concentration
Plasmids
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae Proteins*
Sorbitol
Transcription Factors*
Transformation, Genetic
beta-Galactosidase / genetics

Substances

Culture Media
DNA-Binding Proteins
Fungal Proteins
GAL4 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Sorbitol
DNA
beta-Galactosidase