The result suggests that the cholagogic effect of A. anethoides, A. haichowenesis and A. capillaris are not different significantly (P > 0.05), but the duration of action caused by anethoides and haichowenesis is longer than that caused capillaris. The cholagogic effect of A. capillaris collected in seedling, flower budding (beginning of autumn) and preflower periods were also investigated separately, and the preflower collection was found more cholagogic.