Abstract
Objective:
To investigate the regulation of the prostaglandin (PG) synthesis by interleukin 1 (IL-1) in human synovial cells and chondrocytes.
Methods:
Both cell types stimulated by human recombinant IL-1 synthesized PGE2, PGF2 alpha and 6-keto-PGF1 alpha.
Results:
PGE2 was the major PG synthesized. When arachidonic acid was added exogenously at the end of the stimulation, an increase in the prostaglandin synthesis was observed after 6 and 24 h suggesting that cyclooxygenase is the limiting enzyme. Using actinomycin D and cycloheximide, PG synthesis was shown to be protein synthesis dependent. Inhibition of the constitutive cyclooxygenase by aspirin before the IL-1 stimulation confirmed that the increased prostaglandin synthesis was due to a de novo synthesis of cyclooxygenase.
Conclusion:
This enzyme induction by IL-1 was found to be similar in both cell types.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachidonic Acid / pharmacology
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Aspirin / pharmacology
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Cartilage / drug effects*
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Cartilage / enzymology
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Cells, Cultured
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Cyclophosphamide / pharmacology
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Dactinomycin / pharmacology
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Dinoprostone / biosynthesis
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Enzyme Induction / drug effects
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Humans
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Interleukin-1 / pharmacology*
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Phospholipases A / metabolism
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Prostaglandin-Endoperoxide Synthases / biosynthesis*
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Prostaglandin-Endoperoxide Synthases / genetics
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Prostaglandins / biosynthesis*
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Protein Biosynthesis / drug effects
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Recombinant Proteins / pharmacology
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Synovial Membrane / drug effects*
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Synovial Membrane / enzymology
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Transcription, Genetic / drug effects
Substances
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Interleukin-1
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Prostaglandins
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Recombinant Proteins
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Dactinomycin
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Arachidonic Acid
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Cyclophosphamide
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Prostaglandin-Endoperoxide Synthases
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Phospholipases A
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Dinoprostone
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Aspirin