Non-systemic expression of a stress-responsive maize polyubiquitin gene (Ubi-1) in transgenic rice plants

Plant Mol Biol. 1994 Nov;26(3):1007-12. doi: 10.1007/BF00028868.

Abstract

We have used the promoter, 1st exon and 1st intron of the maize polyubiquitin gene (Ubi-1) for rice transformation experiments and revealed the characteristic expression of Ubi-1 gene: (1) Ubi-1 gene is not regulated systemically but rather individual cells respond independently to the heat or physical stress; (2) Ubi-1 gene changes its tissue-specific expression in response to stress treatment; (3) the expression of Ubi-1 gene is dependent on cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetyltransferases / biosynthesis
  • Acetyltransferases / genetics
  • Adaptation, Physiological / genetics
  • Biopolymers / biosynthesis
  • Biopolymers / genetics*
  • Exons / genetics
  • Gene Expression Regulation, Plant*
  • Genes, Plant / genetics*
  • Glucuronidase / biosynthesis
  • Glucuronidase / genetics
  • Herbicides / pharmacology
  • Hot Temperature
  • Introns / genetics
  • Organophosphorus Compounds / pharmacology
  • Oryza / drug effects
  • Oryza / genetics
  • Oryza / metabolism*
  • Plant Shoots / metabolism
  • Plants, Genetically Modified / drug effects
  • Polyubiquitin
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins / biosynthesis
  • Tissue Distribution
  • Ubiquitins / biosynthesis
  • Ubiquitins / genetics*
  • Zea mays / genetics*

Substances

  • Biopolymers
  • Herbicides
  • Organophosphorus Compounds
  • Recombinant Fusion Proteins
  • Ubiquitins
  • Polyubiquitin
  • bialaphos
  • Acetyltransferases
  • phosphinothricin N-acetyltransferase
  • Glucuronidase