Human recombinant interleukin-1 receptor antagonist blocks bone resorption induced by interleukin-1 beta but not interleukin-1 alpha

Calcif Tissue Int. 1994 Jul;55(1):12-5. doi: 10.1007/BF00310162.

Abstract

Both interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are powerful stimulators of bone resorption in vivo and in vitro. Interleukin-1 receptor antagonist (IL-1ra) binds to many interleukin-1 receptors. It does not activate the receptor and effectively blocks the action of IL-1 alpha and IL-1 beta. In this study, human recombinant IL-1ra, at 100-fold excess, was found to block bone resorption in cultured mouse calvaria due to IL-1 beta but not IL-1 alpha. These observations may be explained by differential affinities of receptors for IL-1 alpha, IL-1 beta and rhIL-1ra on target bone cells.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Bone Resorption / chemically induced
  • Bone Resorption / prevention & control*
  • Cells, Cultured
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / toxicity*
  • Mice
  • Osteoclasts / drug effects*
  • Parathyroid Hormone / toxicity
  • Peptide Fragments / toxicity
  • Receptors, Interleukin-1 / antagonists & inhibitors*
  • Recombinant Proteins / pharmacology
  • Recombinant Proteins / therapeutic use
  • Sialoglycoproteins / pharmacology
  • Sialoglycoproteins / therapeutic use*
  • Teriparatide

Substances

  • IL1RN protein, human
  • Il1rn protein, mouse
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Parathyroid Hormone
  • Peptide Fragments
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Sialoglycoproteins
  • Teriparatide