Restriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast-growing mycobacterial species

FEMS Microbiol Lett. 1994 Feb 1;116(1):19-24. doi: 10.1111/j.1574-6968.1994.tb06669.x.

Abstract

DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA-DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast-growing mycobacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial
  • Genes, Bacterial
  • Molecular Sequence Data
  • Mycobacterium / genetics*
  • Mycobacterium / growth & development
  • Mycobacterium chelonae / genetics
  • Mycobacterium chelonae / growth & development
  • Polymorphism, Restriction Fragment Length*
  • RNA, Ribosomal, 16S / genetics*
  • Time Factors

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S