Abstract
A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Coleoptera / enzymology
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Coleoptera / genetics*
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DNA Transposable Elements
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Escherichia coli / genetics
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Gene Amplification
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Gene Expression
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Genes, Insect*
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Genes, Reporter
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Genetic Markers
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Genetic Techniques
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Genetic Vectors
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Luciferases / genetics*
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Luminescent Measurements
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Nucleic Acid Hybridization
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Plasmids / genetics*
Substances
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DNA Transposable Elements
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Genetic Markers
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Luciferases