Use of firefly luciferase gene for plasmid copy number determination

Plasmid. 1994 Nov;32(3):336-41. doi: 10.1006/plas.1994.1074.

Abstract

A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coleoptera / enzymology
  • Coleoptera / genetics*
  • DNA Transposable Elements
  • Escherichia coli / genetics
  • Gene Amplification
  • Gene Expression
  • Genes, Insect*
  • Genes, Reporter
  • Genetic Markers
  • Genetic Techniques
  • Genetic Vectors
  • Luciferases / genetics*
  • Luminescent Measurements
  • Nucleic Acid Hybridization
  • Plasmids / genetics*

Substances

  • DNA Transposable Elements
  • Genetic Markers
  • Luciferases