A new method for direct analysis of polymerase chain reaction-amplified human papillomavirus using DNA enzyme immunoassay

F Sangiuolo; L De Santis; A Cavicchini; U Angeloni; C Romanini; G Novelli; B Dallapiccola

doi:10.1007/BF02592468

A new method for direct analysis of polymerase chain reaction-amplified human papillomavirus using DNA enzyme immunoassay

Int J Clin Lab Res. 1994;24(4):223-6. doi: 10.1007/BF02592468.

Authors

F Sangiuolo¹, L De Santis, A Cavicchini, U Angeloni, C Romanini, G Novelli, B Dallapiccola

Affiliation

¹ Department of Human Genetics, Tor Vergata University of Rome, Italy.

PMID: 7894049
DOI: 10.1007/BF02592468

Abstract

We describe use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of multiple types of human papillomavirus in a single microtiter plate. The method is specific, sensitive, rapid, and capable of detecting human papillomavirus-6, -11, -16, -18, and -33 in cervical biopsy specimens.

MeSH terms

Base Sequence
DNA Probes, HPV
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Molecular Sequence Data
Papillomaviridae / isolation & purification*
Polymerase Chain Reaction / methods*

Substances

DNA Probes, HPV