Optimization of quantitative culture assay for human immunodeficiency virus from plasma. Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases)

J L Lathey; S A Fiscus; S Rasheed; J C Kappes; B P Griffith; T Elbeik; S A Spector; P S Reichelderfer

doi:10.1128/jcm.32.12.3064-3067.1994

Optimization of quantitative culture assay for human immunodeficiency virus from plasma. Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases)

J Clin Microbiol. 1994 Dec;32(12):3064-7. doi: 10.1128/jcm.32.12.3064-3067.1994.

Authors

J L Lathey¹, S A Fiscus, S Rasheed, J C Kappes, B P Griffith, T Elbeik, S A Spector, P S Reichelderfer

Affiliation

¹ Department of Pediatrics, University of California, San Diego, La Jolla 92093.

Abstract

Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Blood / virology
Citric Acid*
Glucose / analogs & derivatives
HIV Seropositivity / virology
HIV-1 / isolation & purification*
Humans
Microbiological Techniques*
Reproducibility of Results

Substances

acid citrate dextrose
Citric Acid
Glucose

Abstract

Publication types

MeSH terms

Substances

Grants and funding