A sample of 596 women was examined by the direct immunofluorescent test (DIF) for the detection of chlamydia brachomatis antigens in the endocervical smear (with monoclonal antibodies), ELISA test for the detection of IgG antibodies against the group chlamydia-antigen. The chlamydia trachomatis infection was found in 30.20% of the cases, with a high positive rate in all age groups. These infections were more frequently found in women in whom sterility had been diagnosed, compared to other examined women. In relation to other inflammatory processes the significantly highest rate was found in women with changes in the cervix (endocervicitis and arythroplakia). A high rate of chlamydia positive (42.44%) was detected in women who had had pathological pregnancies and deliveries. Humoral immune response to the presence of chlamydia trachomatis infection was proved in 79.44% of the cases. We showed and analyzed diagnostic possibilities of the used diagnostic tests where a combination of the DIF test and ELISA was found the best. For a successful diagnosis two methods have been recommended: a direct one-for the detection of the cause (DIF test, isolation in the cell culture McCoy or Hela 229) and an indirect one for the detection of specific antibodies in the serum.