Abstract
Polyclonal antibodies catalyzing the hydrolysis of carbonate ester were generated by immunizing a rabbit with hapten(4-nitrophenyl phosphate II) conjugated to keyhole limpet hemocyanin. The hydrolytic activity of IgG purified from antisera exhibited plateu one month later than the simple hapten-binding. The affinity of IgG with substrate increased even after the hapten-binding reached plateu. These suggest a strategy to generate good polyclonal catalytic antibodies and the day to fuse spleen cell with myeloma cell to get good monoclonal catalytic antibodies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / metabolism
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Animals
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Binding, Competitive
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Carbonates / metabolism
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Female
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Haptens / administration & dosage
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Haptens / immunology
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Haptens / metabolism*
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Hemocyanins / administration & dosage
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Hemocyanins / immunology
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Hemocyanins / metabolism
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Hydrolysis
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Immune Sera / immunology
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Immunoglobulin Fab Fragments / immunology
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Immunoglobulin G / biosynthesis
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Immunoglobulin G / metabolism*
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Immunoglobulin M / biosynthesis
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Immunoglobulin M / metabolism
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Kinetics
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Nitrophenols
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Organophosphonates / administration & dosage
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Organophosphonates / immunology
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Organophosphonates / metabolism*
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Rabbits
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Spectrophotometry, Ultraviolet
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Spleen / cytology
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Vaccination
Substances
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Adjuvants, Immunologic
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Carbonates
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Haptens
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Immune Sera
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Immunoglobulin M
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Nitrophenols
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Organophosphonates
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4-nitrophenyl phosphonate
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Hemocyanins
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keyhole-limpet hemocyanin