Differential effects of protein phosphatase 2A on distinguishable intracellular signals initiated by v-Src and mediated by HaRas

Biochem Biophys Res Commun. 1994 Dec 15;205(2):1043-50. doi: 10.1006/bbrc.1994.2771.

Abstract

v-Src activates gene expression mediated by serum response elements (SREs) and TPA response elements (TREs). v-Src-induced SRE- and TRE-mediated gene expressions are both dependent upon HaRas. Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase that has been implicated in v-Src-initiated signals. We report here that expression of the catalytic subunit of PP2A upregulates v-Src- and v-HaRas-induced TRE-mediated gene expression, whereas PP2A downregulates v-Src- and v-HaRas-induced SRE-mediated gene expression. These data suggest that intracellular signals activated by v-Src and mediated by HaRas are differentially regulated by PP2A.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • Cloning, Molecular
  • Gene Expression Regulation
  • Mice
  • Oncogene Protein p21(ras) / metabolism*
  • Oncogene Protein pp60(v-src) / metabolism*
  • Phosphoprotein Phosphatases / metabolism*
  • Plasmids
  • Protein Phosphatase 2
  • Recombinant Proteins / biosynthesis
  • Signal Transduction*
  • Transfection

Substances

  • Recombinant Proteins
  • Chloramphenicol O-Acetyltransferase
  • Oncogene Protein pp60(v-src)
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2
  • Oncogene Protein p21(ras)