Insulin-mediated activation of glycogen synthase in isolated skeletal muscle: role of mitochondrial respiration

Biochim Biophys Acta. 1995 May 11;1244(1):229-32. doi: 10.1016/0304-4165(95)00050-l.

Abstract

Activation of glycogen synthase (GS) by insulin in isolated, aerated muscle was abolished when oxygen was replaced by nitrogen. Reoxygenation of the muscle after prior exposure to nitrogen restored the insulin effect. In the presence of cyanide (inhibitor of mitochondrial respiration) insulin did not activate GS. These data demonstrate that adequate mitochondrial respiration is required for insulin-mediated activation of GS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Cyanides / pharmacology
  • Enzyme Activation / drug effects
  • Glycogen / metabolism
  • Glycogen Synthase / metabolism*
  • Insulin / pharmacology*
  • Male
  • Mice
  • Mitochondria, Muscle / metabolism*
  • Muscles / metabolism*
  • Nitrogen / pharmacology
  • Oxygen Consumption
  • Phosphocreatine / metabolism

Substances

  • Cyanides
  • Insulin
  • Phosphocreatine
  • Adenosine Triphosphate
  • Glycogen
  • Glycogen Synthase
  • Nitrogen