Abstract
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP-1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied. However, type I and III collagen expressions varied parallely. All-trans retinoic acid (RA) decreased collagenase expression and stimulated TIMP-1 expression. Under RA action, high variability in mRNAs levels encoding type I and III collagens was observed with HSF passages. However, RA tended to correct variations in collagens expressions observed along HSF life span.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Division / drug effects
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Cellular Senescence / drug effects
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Cellular Senescence / genetics
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Collagen / drug effects*
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Collagen / genetics
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Collagenases / drug effects*
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Collagenases / genetics
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Fibroblasts / chemistry
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Fibroblasts / drug effects*
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Gene Expression Regulation / drug effects
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Glycoproteins / drug effects*
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Glycoproteins / genetics
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Humans
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Matrix Metalloproteinase Inhibitors
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RNA, Messenger / analysis
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Skin / cytology
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Tissue Inhibitor of Metalloproteinases
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Tretinoin / pharmacology
Substances
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Glycoproteins
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Matrix Metalloproteinase Inhibitors
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinases
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Tretinoin
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Collagen
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Collagenases