Uncoordinate expressions of type I and III collagens, collagenase and tissue inhibitor of matrix metalloproteinase 1 along in vitro proliferative life span of human skin fibroblasts. Regulation by all-trans retinoic acid

Cell Biol Int. 1995 Feb;19(2):129-35. doi: 10.1006/cbir.1995.1053.

Abstract

In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP-1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied. However, type I and III collagen expressions varied parallely. All-trans retinoic acid (RA) decreased collagenase expression and stimulated TIMP-1 expression. Under RA action, high variability in mRNAs levels encoding type I and III collagens was observed with HSF passages. However, RA tended to correct variations in collagens expressions observed along HSF life span.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Cellular Senescence / drug effects
  • Cellular Senescence / genetics
  • Collagen / drug effects*
  • Collagen / genetics
  • Collagenases / drug effects*
  • Collagenases / genetics
  • Fibroblasts / chemistry
  • Fibroblasts / drug effects*
  • Gene Expression Regulation / drug effects
  • Glycoproteins / drug effects*
  • Glycoproteins / genetics
  • Humans
  • Matrix Metalloproteinase Inhibitors
  • RNA, Messenger / analysis
  • Skin / cytology
  • Tissue Inhibitor of Metalloproteinases
  • Tretinoin / pharmacology

Substances

  • Glycoproteins
  • Matrix Metalloproteinase Inhibitors
  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinases
  • Tretinoin
  • Collagen
  • Collagenases