3-Aminopicolinic acid as a matrix for laser desorption mass spectrometry of biopolymers

Rapid Commun Mass Spectrom. 1994 Dec;8(12):1001-6. doi: 10.1002/rcm.1290081219.

Abstract

3-Aminopicolinic acid (3-APA) was tested and found to be a useful matrix for matrix-assisted laser desorption/ionization of DNA and protein. Single-stranded DNA segments of 150-mer and double-stranded DNA of 246 base pairs were successfully detected by using 3-APA as an ultraviolet-absorbing matrix in a linear time-of-flight mass spectrometer. In the case of the double-stranded DNA, only parent ions corresponding to single-stranded DNA were observed. The comparison with 3-hydroxypicolinic acid and picolinic acid matrices is discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA / analysis*
  • Mass Spectrometry
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Oligonucleotides / analysis
  • Picolinic Acids / analysis*
  • Polymerase Chain Reaction

Substances

  • Oligonucleotides
  • Picolinic Acids
  • 3-aminopicolinic acid
  • DNA