Abstract
Non-charge- and charge-sensitive dextran/polyethyleneglycol two-phase systems have been used to study the surface properties of red blood cells stabilized by crosslinking with dimethyl suberimidate and permeabilized with digitonin. While crosslinked red blood cells show similar hydrophobic- and charge-related surface properties as control cells, the digitonin treatment changes their surface properties. The changes in hydrophobicity are related to the cell total lipid and cholesterol content while the changes in the charge are related to the sialic acid released by neuraminidase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chemical Phenomena
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Chemistry, Physical
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Cholesterol / blood
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Cholesterol / chemistry
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Cross-Linking Reagents / pharmacology
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Dextrans
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Digitonin / pharmacology
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Dimethyl Suberimidate / pharmacology
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Erythrocyte Membrane / chemistry
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Erythrocyte Membrane / drug effects
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Erythrocytes / chemistry*
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Erythrocytes / drug effects
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Lipids / blood
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Lipids / chemistry
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Male
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N-Acetylneuraminic Acid
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Neuraminidase / pharmacology
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Polyethylene Glycols
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Rats
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Rats, Wistar / blood
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Sialic Acids / chemistry
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Sialic Acids / classification
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Surface Properties
Substances
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Cross-Linking Reagents
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Dextrans
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Lipids
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Sialic Acids
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Dimethyl Suberimidate
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Polyethylene Glycols
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Cholesterol
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Neuraminidase
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N-Acetylneuraminic Acid
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Digitonin