Cloning of cDNA encoding rat phospholipase C-beta 4, a new member of the phospholipase C

Biochem Biophys Res Commun. 1993 Jul 30;194(2):706-12. doi: 10.1006/bbrc.1993.1879.

Abstract

Phospholipase C-beta 4(PLC-beta 4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-beta 4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-beta 1, human PLC-beta 2, and rat PLC-beta 3, respectively. The amino acid sequence of PLC-beta 4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-beta subtypes, suggesting that the PLC-beta 4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain / enzymology*
  • Cattle
  • Cerebellum / enzymology
  • Cloning, Molecular
  • DNA
  • Humans
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Poly A / genetics
  • Poly A / isolation & purification
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Rats
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Type C Phospholipases / biosynthesis
  • Type C Phospholipases / genetics*

Substances

  • Isoenzymes
  • RNA, Messenger
  • Poly A
  • RNA
  • DNA
  • Type C Phospholipases

Associated data

  • GENBANK/D16316
  • GENBANK/D31661
  • GENBANK/D37827
  • GENBANK/L16877
  • GENBANK/L16878
  • GENBANK/L18962
  • GENBANK/L19108
  • GENBANK/L19109
  • GENBANK/L19111
  • GENBANK/L19112