Abstract
Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Calcium / metabolism
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Calcium / pharmacology*
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Charybdotoxin
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DNA / genetics
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Drosophila
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Electric Conductivity
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Large-Conductance Calcium-Activated Potassium Channels
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Mice
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Molecular Sequence Data
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Oocytes / metabolism
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Peptides / pharmacology
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Potassium / metabolism
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Potassium Channels / chemistry
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Potassium Channels / drug effects
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Potassium Channels / genetics*
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Potassium Channels / metabolism
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Potassium Channels, Calcium-Activated*
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RNA / genetics
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RNA, Complementary
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Scorpion Venoms / pharmacology
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Sodium / metabolism
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Tetraethylammonium
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Tetraethylammonium Compounds / pharmacology
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Transcription, Genetic
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Xenopus
Substances
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Large-Conductance Calcium-Activated Potassium Channels
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Membrane Proteins
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Peptides
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Potassium Channels
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Potassium Channels, Calcium-Activated
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RNA, Complementary
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Scorpion Venoms
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Tetraethylammonium Compounds
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Charybdotoxin
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RNA
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Tetraethylammonium
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iberiotoxin
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DNA
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Sodium
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Potassium
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Calcium