Specific regions of Escherichia coli OmpF protein involved in antigenic and colicin receptor sites and in stable trimerization

Abstract

Four different mutations were obtained by selecting for resistance to colicin N and screening for continued production of the OmpF protein of Escherichia coli. Two of them also conferred resistance to colicin A. The substitutions C for R-168 (R168C) and E284K caused the loss of the E21 epitope, while the transition G285D altered the E18, E19, and E20 antigenic sites. The substitution G119D drastically affected the stability of the trimeric conformation.