NAD(+)-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

T Karasawa; S Takasawa; K Yamakawa; H Yonekura; H Okamoto; S Nakamura

doi:10.1111/j.1574-6968.1995.tb07720.x

NAD(+)-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

FEMS Microbiol Lett. 1995 Aug 1;130(2-3):201-4. doi: 10.1111/j.1574-6968.1995.tb07720.x.

Authors

T Karasawa¹, S Takasawa, K Yamakawa, H Yonekura, H Okamoto, S Nakamura

Affiliation

¹ Department of Bacteriology, School of Medicine, Kanazawa University, Japan.

PMID: 7649441
DOI: 10.1111/j.1574-6968.1995.tb07720.x

Abstract

NAD(+)-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Diphosphate Ribose / analogs & derivatives*
Adenosine Diphosphate Ribose / biosynthesis
Cyclic ADP-Ribose
NAD+ Nucleosidase / isolation & purification
NAD+ Nucleosidase / pharmacology*
Streptococcus pyogenes / enzymology*

Substances

Cyclic ADP-Ribose
Adenosine Diphosphate Ribose
NAD+ Nucleosidase