A 67-kDa protein binding to the curved DNA in the main regulatory region of the rat mitochondrial genome

Gene. 1995 Jul 28;160(2):229-34. doi: 10.1016/0378-1119(95)00122-m.

Abstract

We have purified, by sequence-specific affinity chromatography, a mitochondrial (mt) matrix protein which binds to the curved DNA located between the replication origin (ori) of the leading strand (ori-H) and the two transcription promoters in the rat mt genome. The protein was characterized by gel electrophoresis as a 67-kDa polypeptide and seems to be involved in the DNA contact on the mt light strand. This protein differs (in the size and location of its DNA-binding site) from other DNA-binding proteins studied so far in animal mt systems. We suggest a role for the 67-kDa protein, assisted by other proteins, in regulating the initiation of leading-strand replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Mitochondrial / genetics
  • DNA, Mitochondrial / metabolism*
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology
  • Mitochondria, Liver / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Binding
  • Rats / genetics
  • Rats / metabolism*
  • Rats, Wistar
  • Regulatory Sequences, Nucleic Acid*
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription Factors / physiology

Substances

  • DNA, Mitochondrial
  • DNA-Binding Proteins
  • Transcription Factors
  • mitochondrial matrix protein, 67-kDa, rat