Abstract
We have purified, by sequence-specific affinity chromatography, a mitochondrial (mt) matrix protein which binds to the curved DNA located between the replication origin (ori) of the leading strand (ori-H) and the two transcription promoters in the rat mt genome. The protein was characterized by gel electrophoresis as a 67-kDa polypeptide and seems to be involved in the DNA contact on the mt light strand. This protein differs (in the size and location of its DNA-binding site) from other DNA-binding proteins studied so far in animal mt systems. We suggest a role for the 67-kDa protein, assisted by other proteins, in regulating the initiation of leading-strand replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA, Mitochondrial / genetics
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DNA, Mitochondrial / metabolism*
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology
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Mitochondria, Liver / metabolism*
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Molecular Sequence Data
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Nucleic Acid Conformation
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Protein Binding
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Rats / genetics
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Rats / metabolism*
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Rats, Wistar
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Regulatory Sequences, Nucleic Acid*
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Transcription Factors / isolation & purification*
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Transcription Factors / metabolism
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Transcription Factors / physiology
Substances
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DNA, Mitochondrial
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DNA-Binding Proteins
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Transcription Factors
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mitochondrial matrix protein, 67-kDa, rat