Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome

Cytogenet Cell Genet. 1995;71(1):96-8. doi: 10.1159/000134071.

Abstract

We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azo Compounds
  • Cells, Cultured
  • Chromosome Banding / methods*
  • Fluorescent Dyes*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Mice
  • Naphthalenes*

Substances

  • Azo Compounds
  • Fluorescent Dyes
  • Naphthalenes
  • 3-hydroxy-N-(2'-biphenyl)-2-naphthalenecarboxamide phosphate