Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis

P Huberts; V Mizrahi

doi:10.1016/0378-1119(95)00453-d

Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis

Gene. 1995 Oct 16;164(1):133-6. doi: 10.1016/0378-1119(95)00453-d.

Authors

P Huberts¹, V Mizrahi

Affiliation

¹ Department of Hematology, University of the Witwatersrand Medical School, Johannesburg, South Africa.

PMID: 7590302
DOI: 10.1016/0378-1119(95)00453-d

Abstract

The polA gene (encoding DNA polymerase I) from Mycobacterium tuberculosis was cloned using an internal gene segment probe generated by PCR amplification of genomic DNA [Mizrahi et al., Gene 136 (1993) 287-290]. The gene encodes a polypeptide 904 amino acids (aa) in length that shares 89% identity with a 911-aa homologue from Mycobacterium leprae. The polypeptide has all of the primary structural elements necessary for DNA polymerase and 5'-3' exonuclease activity, but lacks the motifs required for an associated 3'-5' exonuclease (proofreading) activity.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cloning, Molecular / methods
DNA Polymerase I / genetics*
Genes, Bacterial*
Molecular Sequence Data
Mycobacterium tuberculosis / enzymology
Mycobacterium tuberculosis / genetics*
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid

Substances

DNA Polymerase I

Associated data

GENBANK/L11920