Abstract
Sixteen methacarn-fixed and paraffin-embedded cervical squamous lesions were examined using monoclonal antibodies to p53 and MDM2 in order to compare the expression of both proteins in cervical neoplasia. Standard avidin-biotin or streptavidin-biotin immunoperoxidase techniques were employed. The results show that the overexpression of both proteins takes place in a meaningful proportion of cervical neoplastic lesions. The expression of either protein is not very frequent in CIN 1 and 2. The overexpression of either p53 (9/12) or MDM2 (4/10) proteins was recorded in the group of more advanced lesions, their level fluctuating from 10% to 60% positive cells. The results suggest the possibility of an interaction of the p53 and MDM2 proteins in some cases of cervical neoplasia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates
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Acetic Acid*
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Antibodies, Monoclonal
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology*
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Chloroform
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Female
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Fixatives
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Gene Expression*
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Histological Techniques
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Humans
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Methanol
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Neoplasm Proteins / analysis
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Neoplasm Proteins / biosynthesis
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Nuclear Proteins*
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Paraffin
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins c-mdm2
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Tumor Suppressor Protein p53 / analysis
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Tumor Suppressor Protein p53 / biosynthesis*
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Uterine Cervical Neoplasms / metabolism
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Uterine Cervical Neoplasms / pathology*
Substances
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Acetates
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Antibodies, Monoclonal
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Fixatives
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Neoplasm Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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Tumor Suppressor Protein p53
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methacarn
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Chloroform
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Paraffin
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MDM2 protein, human
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Proto-Oncogene Proteins c-mdm2
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Acetic Acid
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Methanol