Functional expression of the human CHIP28 water channel in a yeast secretory mutant

V Laizé; G Rousselet; J M Verbavatz; V Berthonaud; R Gobin; N Roudier; L Abrami; P Ripoche; F Tacnet

doi:10.1016/0014-5793(95)01060-r

Functional expression of the human CHIP28 water channel in a yeast secretory mutant

FEBS Lett. 1995 Oct 16;373(3):269-74. doi: 10.1016/0014-5793(95)01060-r.

Authors

V Laizé¹, G Rousselet, J M Verbavatz, V Berthonaud, R Gobin, N Roudier, L Abrami, P Ripoche, F Tacnet

Affiliation

¹ Département de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, CEA-Saclay, Gif sur Yvette, France.

PMID: 7589481
DOI: 10.1016/0014-5793(95)01060-r

Abstract

The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.

MeSH terms

4-Chloromercuribenzenesulfonate / pharmacology
Aquaporin 1
Aquaporins*
Base Sequence
Blood Group Antigens
Cytoplasmic Granules / metabolism
Cytoplasmic Granules / ultrastructure
Fluorescent Antibody Technique
Freeze Fracturing
Humans
Ion Channels / analysis
Ion Channels / genetics*
Ion Channels / metabolism
Kinetics
Microscopy, Electron
Molecular Sequence Data
Mutation
Osmolar Concentration
Recombinant Proteins
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Sorbitol / pharmacology
Temperature
Transformation, Genetic
Water / metabolism

Substances

AQP1 protein, human
Aquaporins
Blood Group Antigens
Ion Channels
Recombinant Proteins
Water
Aquaporin 1
Sorbitol
4-Chloromercuribenzenesulfonate