Abstract
We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. With these constructions, we were able to study the role of these isoenzymes in pyruvate biosynthesis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cloning, Molecular
-
Escherichia coli / enzymology*
-
Escherichia coli / genetics
-
Escherichia coli / growth & development
-
Genes, Bacterial / genetics*
-
Gluconates / metabolism
-
Glucose / metabolism
-
Isoenzymes / genetics*
-
Isoenzymes / metabolism
-
Mutation
-
Pyruvate Kinase / genetics*
-
Pyruvate Kinase / metabolism
-
Pyruvates / metabolism*
-
Pyruvic Acid
-
Ribose / metabolism
-
Sequence Analysis, DNA
Substances
-
Gluconates
-
Isoenzymes
-
Pyruvates
-
Ribose
-
Pyruvic Acid
-
Pyruvate Kinase
-
Glucose
-
gluconic acid