Abstract
Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA became much less dependent on a nitrogen source-associated signal.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology
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Bacillus subtilis / genetics*
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Bacterial Proteins*
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Base Sequence
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Gene Expression Regulation, Bacterial / genetics*
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Genes, Bacterial / genetics
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Glutamate Synthase / biosynthesis
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Glutamate Synthase / genetics*
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Molecular Sequence Data
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Mutation
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Promoter Regions, Genetic / genetics
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Recombinant Fusion Proteins / biosynthesis
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Repressor Proteins / chemistry
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Repressor Proteins / genetics*
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Trans-Activators / chemistry
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Trans-Activators / genetics*
Substances
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Bacterial Proteins
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GltC protein, Bacillus subtilis
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Recombinant Fusion Proteins
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Repressor Proteins
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Trans-Activators
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Glutamate Synthase