Abstract
The role of syntaxin 1, a protein involved in the docking of synaptic vesicles at presynaptic active zones, has been investigated in pancreatic islet cells. Using two different monoclonal antibodies we have shown that syntaxin 1 is present in the pancreatic islet cell microsomal fraction. Furthermore, functional experiments demonstrate that anti-syntaxin antibodies inhibit CA(2+)-dependent insulin secretion in permeabilized islet cells. These data indicate that syntaxin 1 is present in the pancreatic beta cell and it is likely to play a functional role in the exocytosis of secretory granules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / pharmacology*
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Antigens, Surface / analysis
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Antigens, Surface / immunology
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Antigens, Surface / physiology*
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Calcium / pharmacology
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Cytoplasmic Granules / physiology
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Exocytosis
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Immunohistochemistry
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Insulin / metabolism*
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Insulin Secretion
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Islets of Langerhans / metabolism
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Islets of Langerhans / physiology*
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Islets of Langerhans / ultrastructure
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Male
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Mice
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Microsomes / physiology
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / immunology
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Nerve Tissue Proteins / physiology*
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Syntaxin 1
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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Insulin
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Nerve Tissue Proteins
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Stx1a protein, mouse
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Syntaxin 1
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Calcium