Rapid detection and identification of Vibrio anguillarum by using a specific oligonucleotide probe complementary to 16S rRNA

J Martínez-Picado; A R Blanch; J Jofre

doi:10.1128/aem.60.2.732-737.1994

Rapid detection and identification of Vibrio anguillarum by using a specific oligonucleotide probe complementary to 16S rRNA

Appl Environ Microbiol. 1994 Feb;60(2):732-7. doi: 10.1128/aem.60.2.732-737.1994.

Authors

J Martínez-Picado¹, A R Blanch, J Jofre

Affiliation

¹ Department of Microbiology, University of Barcelona, Spain.

Abstract

Partial 16S rDNA from Vibrio collection type strains and recent isolates of Vibrio-related strains were sequenced and compared with previously published sequences. A 24-base DNA oligonucleotide (VaV3) was designed and used as a specific probe for detection and identification of Vibrio anguillarum. Its specificity was tested against collection type strains and environmental isolates and no cross-reaction was found. The probe detected 8 of the 10 V. anguillarum serovars. It was applied to screen different Vibrio-related strains isolated from marine hatcheries and fish farms. The detection limit in DNA-DNA slot blot hybridization was 150 pg.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Molecular Sequence Data
Oligonucleotide Probes
RNA, Bacterial / genetics
Sensitivity and Specificity
Species Specificity
Time Factors
Vibrio / genetics
Vibrio / isolation & purification*

Substances

DNA, Bacterial
DNA, Ribosomal
Oligonucleotide Probes
RNA, Bacterial