Inhibition of inducible nitric oxide synthase mRNA expression by basic fibroblast growth factor in human microglial cells

Neurosci Lett. 1995 Jul 28;195(1):45-8. doi: 10.1016/0304-3940(95)11778-u.

Abstract

The effect of basic fibroblast growth factor (bFGF) on inducible nitric oxide synthase (iNOS) mRNA expression in human cultured ramified microglial cells was investigated. Using RT-PCR and Southern blot analysis, we found that bFGF prevented the iNOS gene expression as induced by LPS/TNF alpha. Also, bFGF dose-dependently inhibited nitrite levels in treated cell supernatants. That the early presence of bFGF during LPS/TNF alpha induction was essential indicates that iNOS gene expression can be transcriptionally regulated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Cells, Cultured
  • Enzyme Induction / drug effects
  • Fibroblast Growth Factor 2 / pharmacology*
  • Humans
  • Microglia / drug effects
  • Microglia / metabolism*
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Nitric Oxide Synthase / biosynthesis
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis*

Substances

  • RNA, Messenger
  • Fibroblast Growth Factor 2
  • Nitric Oxide Synthase