The relationship between the minimal and the 50% haemolytic dose in complement titrations

Onderstepoort J Vet Res. 1981 Dec;48(4):259-60.

Abstract

The 50% spectrophotometric complement titration end-point (C'H50) was found to be more reproducible than the 100% end-point. The relationship of the 100% end-point (MHD) to the (C'H50) was 1:2, 5. The use of thrombin tubes induced clotting within 2 min and enabled the process of complement preparation to be completed well within 1 hour.

MeSH terms

  • Animals
  • Complement Fixation Tests / methods*
  • Evaluation Studies as Topic
  • Guinea Pigs
  • Male