Abstract
Amyloglucosidase (glucoamylase; EC 3.2.1.3) has been purified from the culture filtrates of Aspergillus candidus Link var. aureus using hydrophobic interaction chromatography and DEAE-cellulose treatment. The enzyme thus obtained has a specific activity of 329 units/mg protein with the overall recoveries between 70 and 75%. The process appears to be of industrial promise.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aspergillus / enzymology
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Chromatography, Affinity
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Chromatography, Agarose
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Chromatography, DEAE-Cellulose
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Glucan 1,4-alpha-Glucosidase / isolation & purification*
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Glucosidases / isolation & purification*
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Glycine / analogs & derivatives
Substances
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N-phenylglycine
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Glucosidases
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Glucan 1,4-alpha-Glucosidase
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Glycine