Purification of amyloglucosidase

Anal Biochem. 1983 Sep;133(2):482-5. doi: 10.1016/0003-2697(83)90113-6.

Abstract

Amyloglucosidase (glucoamylase; EC 3.2.1.3) has been purified from the culture filtrates of Aspergillus candidus Link var. aureus using hydrophobic interaction chromatography and DEAE-cellulose treatment. The enzyme thus obtained has a specific activity of 329 units/mg protein with the overall recoveries between 70 and 75%. The process appears to be of industrial promise.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology
  • Chromatography, Affinity
  • Chromatography, Agarose
  • Chromatography, DEAE-Cellulose
  • Glucan 1,4-alpha-Glucosidase / isolation & purification*
  • Glucosidases / isolation & purification*
  • Glycine / analogs & derivatives

Substances

  • N-phenylglycine
  • Glucosidases
  • Glucan 1,4-alpha-Glucosidase
  • Glycine