An edeine resistant mRNA-dependent protein synthesis system from a Saccharomyces cerevisiae mutant

FEBS Lett. 1984 Aug 20;174(1):47-9. doi: 10.1016/0014-5793(84)81075-3.

Abstract

A cell-free protein synthesizing system from a mutant of Saccharomyces cerevisiae translated exogenous mRNA in the presence of 2 microM edeine, while a similar system from wild-type strain was completely inhibited by the drug. The mutant ribosomes showed an affinity for [125I]edeine comparable to the wild-type ribosomes, thereby suggesting that these macromolecules alone were not responsible for the edeine-resistant capacity of the mutant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / toxicity*
  • Cell-Free System
  • Edeine / metabolism
  • Edeine / toxicity*
  • Mutation*
  • Protein Biosynthesis / drug effects*
  • RNA, Messenger / genetics*
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Species Specificity

Substances

  • Anti-Bacterial Agents
  • RNA, Messenger
  • Edeine