Complex-formation between the fibrin-derived plasmic fragments DD and E demonstrated by crossed immunoelectrophoresis

Thromb Res. 1983 Jun 1;30(5):441-8. doi: 10.1016/0049-3848(83)90178-0.

Abstract

The formation of a complex between the fibrin fragments DD and E was studied by crossed immunoelectrophoresis using antibodies against human fibrinogen. The complex formation was seen by a common electrophoretic migration of the DD-fragment and part of the E-fragments. This effect was abolished by a further incubation with plasmin of the preparation containing the (DD) E-complex. This also led to an anodal shift in migration of the E-fragment indicating a transfer from E1 to E3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Fibrin / metabolism*
  • Fibrin Fibrinogen Degradation Products / metabolism*
  • Fibrinogen / metabolism
  • Fibrinolysin / pharmacology
  • Humans
  • Immunoelectrophoresis*
  • Immunoelectrophoresis, Two-Dimensional*

Substances

  • Fibrin Fibrinogen Degradation Products
  • fibrinogen D fragment
  • fibrinogen fragment E
  • Fibrin
  • Fibrinogen
  • Fibrinolysin