The antibiotic resistance plasmid pPH1JI was derived from two IncP plasmids, R751 and R1033. The suicide vector for Tn5, pJB4JI, contains pPH1JI, bacteriophage Mu, and Tn5. Restriction enzyme cleavage maps for pPH1JI and pJB4JI, and the antibiotic resistance levels determined by pPH1JI and its parent plasmids are presented. The relationships between pPH1JI and its parent plasmids, and pJB4JI, are discussed.