Pyruvate prevents the ATP depletion caused by formaldehyde or calcium-chelator esters in the human red cell

Biochim Biophys Acta. 1985 Feb 28;813(1):148-50. doi: 10.1016/0005-2736(85)90357-8.

Abstract

Formaldehyde released during hydrolysis of calcium-chelator esters incorporated into cells blocks glycolysis in the human erythrocyte (Tiffert, T., García-Sancho, J. and Lew, V.L. (1984) Biochim. Biophys. Acta 773, 143-156). This blockade is due to the inhibition of glyceraldehyde-3-phosphate dehydrogenase by NAD+ depletion caused by enzymatic oxidation of formaldehyde coupled to NADH production. The addition of pyruvate to the incubation medium prevents or reverts ATP depletion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Aminoquinolines / pharmacology*
  • Erythrocytes / drug effects
  • Erythrocytes / metabolism*
  • Formaldehyde / pharmacology*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Humans
  • In Vitro Techniques
  • NAD / metabolism
  • Pyruvates / pharmacology*
  • Pyruvic Acid

Substances

  • Aminoquinolines
  • Pyruvates
  • NAD
  • Formaldehyde
  • Quin2-acetoxymethyl ester
  • Pyruvic Acid
  • Adenosine Triphosphate
  • Glyceraldehyde-3-Phosphate Dehydrogenases