The serum SHGB (sex-hormone-binding globulin) concentration was measured in patients with secondary amenorrhea in order to determine its role in the regulation of androgen and estrogen metabolism in the course of the normal and irregular menstrual cycle. SHBG concentration was lower than in the control group in 52% of amenorrheic patients, but this difference was not statistically significant due to great individual variations. If the patients were divided in 2 groups according to Prolactin (PRL) concentration, significant decrease in SHGB concentration was observed in the group with elevated PRL concentrations. Correlation of SHBG with estrogen (E2), testosterone (T), progesterone (PRG) and PRL was tested and results show the weak correlation (r = 0.4) between SHGB and T and more significant correlation between SHGB and E2 in the group with basal E2 concentration below 100 pmol/1. SHGB test should be included in the evaluation of alterations of androgen and estrogen metabolism.