Comparative salivary proteomics analysis of children with and without early childhood caries using the DIA approach: A pilot study

Jinxiang Ye; Fangfang Zhang; Zhouyuan Luo; Xiaoyan Ou

doi:10.1002/prca.202400006

Comparative salivary proteomics analysis of children with and without early childhood caries using the DIA approach: A pilot study

Proteomics Clin Appl. 2024 May 20:e2400006. doi: 10.1002/prca.202400006. Online ahead of print.

Authors

Jinxiang Ye¹, Fangfang Zhang¹, Zhouyuan Luo¹, Xiaoyan Ou¹

Affiliation

¹ The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University & Jiangxi Province Key Laboratory of Oral Biomedicine & Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, Jiangxi, China.

PMID: 38769866
DOI: 10.1002/prca.202400006

Abstract

Objective: To screen differentially expressed proteins (DEPs) in the saliva of Early childhood caries (ECC) with different degrees of severity.

Methods: The proteomic profiles of salivary of children with ECC of varying severity by data independent acquisition data independent acquisition (DIA) technique. A total of 12 preschool children aged 3-5 years were included in this study.

Results: In this study, a total of 15,083 peptides and 1944 proteins were quantified; The results of DEPs screening showed that 34 DEPs were identified between the group H and the group LC, including 18 up-regulated proteins and 16 down-regulated proteins; 34 DEPs were screened between the group H and the group HC, including 17 up-regulated proteins and 17 down-regulated proteins; 42 DEPs were screened between the group LC and the group HC, including 18 up-regulated proteins and 24 down-regulated proteins. Among these DEPs, we screened several key proteins that may play a role in ECC, such as MK, histone H4, TGFβ3, ZG16B, MUC20, and SMR-3B.

Conclusion: Salivary proteins, as important host factors of caries, are differentially expressed between the saliva of ECC children and healthy children. Specific DEPs are expected to become potential biomarkers for the diagnosis of ECC.

Keywords: DIA; biomarker; early childhood caries; protein; saliva.