Ultrasound-assisted extraction of polyphenols from lotus rhizome epidermis by alcohol/salt-based aqueous two-phase system: Optimization, extraction mechanism and antioxidant activities

Xianqiang Chen; Yan He; Zhuo Liu; Zhenfu Huang; Chunfang Xu; Yanzhao Liu; Yassin Haran; Waqar Nisar; Shoulei Yan; Jie Li

doi:10.1016/j.foodchem.2024.139620

Ultrasound-assisted extraction of polyphenols from lotus rhizome epidermis by alcohol/salt-based aqueous two-phase system: Optimization, extraction mechanism and antioxidant activities

Food Chem. 2024 May 17:453:139620. doi: 10.1016/j.foodchem.2024.139620. Online ahead of print.

Authors

Xianqiang Chen¹, Yan He¹, Zhuo Liu¹, Zhenfu Huang¹, Chunfang Xu¹, Yanzhao Liu¹, Yassin Haran¹, Waqar Nisar¹, Shoulei Yan², Jie Li³

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
² College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Aquatic Vegetable Preservation and Processing Technology Engineering Center of Hubei Province, Wuhan 430070, China; Hubei Honghu Lotus Rhizome Industry Research Institute, Jingzhou 433299, China. Electronic address: yanshoulei1225@mail.hzau.edu.cn.
³ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Aquatic Vegetable Preservation and Processing Technology Engineering Center of Hubei Province, Wuhan 430070, China.

PMID: 38761727
DOI: 10.1016/j.foodchem.2024.139620

Abstract

In this study, ultrasonic-assisted (UA) alcohol/salt-based aqueous two-phase system (ATPS) method was constructed to extract lotus rhizome epidermis (LRE) polyphenols. The extraction conditions were optimized as salt concentration 26.75 %, ethanol concentration 25.45 %, ultrasonic power 487 W and liquid-solid ratio 35.33 mL/g by comparing response surface methodology (RSM) and artificial neural network (ANN) models. Then, l-dopa (2.35 ± 0.036 mg/g dw), gallocatechin (1.66 ± 0.0035 mg/g dw) and epigallocatechin (1.37 ± 0.0035 mg/g dw) were determined as major polyphenols in LRE by using UA-ATPS method. Moreover, study showed that ultrasound, van der Waals force, hydrogen bond and salting out could accelerate the mass transfer and extraction of polyphenols in LRE cells. The high-pressure cavity and collapse effect of ultrasound could also accelerate the extraction of polyphenols. In vitro antioxidant experiments showed that LRE polyphenols have good antioxidant ability. In sum, this study developed a green and efficient extraction method to enhance the profitability of LRE in food and medicine industries.

Keywords: Antioxidant activity; Artificial neural network; Genetic algorithm; Lotus rhizome epidermis polyphenols; Response surface methodology; Ultrasonic-assisted aqueous two-phase extraction.