Screening for ferroptosis genes related to endometrial carcinoma and predicting of targeted drugs based on bioinformatics

Rui Wang; Wei Lang; Qian Xue; Le Zhang; Yunzhu Xujia; Chaofan Wang; Xin Fang; Shidi Gao; Li Guo

doi:10.1007/s00204-024-03783-6

Screening for ferroptosis genes related to endometrial carcinoma and predicting of targeted drugs based on bioinformatics

Arch Toxicol. 2024 May 17. doi: 10.1007/s00204-024-03783-6. Online ahead of print.

Authors

Rui Wang¹, Wei Lang², Qian Xue¹, Le Zhang¹, Yunzhu Xujia¹, Chaofan Wang¹, Xin Fang¹, Shidi Gao¹, Li Guo³

Affiliations

¹ Department of Toxicology, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
² Department of Gynecology III, Changchun Obstetrics-Gynecology Hospital, Changchun, 130042, Jilin Province, China.
³ Department of Toxicology, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China. gli@jlu.edu.cn.

PMID: 38758406
DOI: 10.1007/s00204-024-03783-6

Abstract

Endometrial carcinoma is one of most common malignant tumors in women, and ferroptosis is closely related to the development and treatment of endometrial carcinoma. The aim of this study was to screen ferroptosis-related genes associated with endometrial carcinoma and predict targeted drugs through bioinformatics. 761 differentially expressed genes were obtained by the dataset GSE63678 from the GEO database, and most of the genes were enriched in the KEGG_CELL_CYCLE and KEGG_OOCYTE_MEIOSIS signaling pathways. 22 ferroptosis-differentially expressed genes were obtained by intersection with the FerrDb database. These genes were involved in biological processes including macromolecular complex assembly and others, and involved in signal pathways including glutathione metabolism, p53 signaling pathway and others. CDKN2A, IDH1, NRAS, TFRC and GOT1 were obtained as hub genes by PPI network analysis. GEPIA showed that CDKN2A, IDH1, NRAS and TFRC were significantly expressed in endometrial carcinoma. Immunohistochemical results showed that CDKN2A, NRAS and TFRC were significantly expressed in endometrial carcinoma clinical tissue samples. The ROC constructed by TCGA database showed that CDKN2A, NRAS and TFRC had significant value in the diagnosis of endometrial carcinoma, and all had prognostic efficacy. 136,572-09-3 BOSS and others were identified as potential targeted drugs for endometrial carcinoma targeting ferroptosis. Our study has shown that ferroptosis-related genes CDKN2A, NRAS and TFRC are diagnostic markers of endometrial carcinoma, and 136,572-09-3 BOSS, methyprylon BOSS, daunorubicin CTD 00005752, nitroglycerin BOSS and dUTP BOSS, IRON BOSS, Imatinib mesylate BOSS, 2-Butanone BOSS, water BOSS, and L-thyroxine BOSS may be potential therapeutic drugs.

Keywords: Bioinformatics; Differentially expressed genes; Drug prediction; Endometrial carcinoma; Ferroptosis.