Extracellular vesicles (EVs) are produced by all domains of life. In fungal pathogens, they participate in virulence mechanisms and/or induce protective immunity, depending on the pathogenic species. EVs produced by pathogenic members of the Cryptococcus genus mediate virulence, antifungal resistance, as well as humoral and cell-mediated immunity. The isolation of cryptococcal EVs has been laborious and time-consuming for years. In this chapter, we detail a fast protocol for the isolation and analysis of EVs produced by members of the Cryptococcus genus.
Keywords: Cryptococcus; Electron microscopy; Extracellular vesicles; Nanoparticle tracking analysis; Solid media.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.