Characterization of the immune suppressive functions of eosinophils

Bomiao Qing; Minyao Li; Dan Peng; Junyi Wang; Shuo Song; Lihua Mo; Guoping Li; Pingchang Yang

doi:10.1016/j.cellimm.2024.104829

Characterization of the immune suppressive functions of eosinophils

Cell Immunol. 2024 May 13:401-402:104829. doi: 10.1016/j.cellimm.2024.104829. Online ahead of print.

Authors

Bomiao Qing¹, Minyao Li², Dan Peng³, Junyi Wang¹, Shuo Song², Lihua Mo², Guoping Li⁴, Pingchang Yang⁵

Affiliations

¹ Laboratory of Allergy and Precision Medicine, Department of Pulmonary and Critical Care Medicine, Chengdu Institute of Respiratory Health, the Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, China.
² Department of General Practice Medicine, Third Affiliated Hospital of Shenzhen University, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University and State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China.
³ Laboratory of Allergy and Precision Medicine, Department of Pulmonary and Critical Care Medicine, Chengdu Institute of Respiratory Health, the Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, China; Department of General Practice Medicine, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
⁴ Laboratory of Allergy and Precision Medicine, Department of Pulmonary and Critical Care Medicine, Chengdu Institute of Respiratory Health, the Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, China. Electronic address: liguoping@swjtu.edu.cn.
⁵ Institute of Allergy & Immunology of Shenzhen University and State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China. Electronic address: pcy2356@163.com.

PMID: 38754338
DOI: 10.1016/j.cellimm.2024.104829

Abstract

Eosinophils account for a significant portion of immune cells in the body. It is well known that eosinophils play a role in the pathogenesis of many diseases. In which the interaction between eosinophils and other immune cells is incompletely understood. The aim of this study is to characterize the immune suppressive functions of eosinophils. In this study, an irway allergy mouse model was established. Eosinophils were isolated from the airway tissues using flow cytometry cell sorting. The RAW264.7 cell line was used to test the immune suppressive functions of eosinophils. We observed that eosinophils had immune suppressive functions manifesting inhibiting immune cell proliferation and cytokine release from other immune cells. The eosinophil's immune suppressive functions were mediated by eosinophil-derived molecules, such as eosinophil peroxidase (EPX) and major basic protein (MBP). The expression of Ras-like protein in the brain 27a (Rab27a) was detected in eosinophils, which controlled the release of MBP and EPX by eosinophils. Eosinophil mediators had two contrast effects on inducing inflammatory responses or rendering immune suppressive effects, depending on the released amounts. Administration of an inhibitor of Rab27a at proper dosage could alleviate experimental airway allergy. To sum up, eosinophils have immune suppressive functions and are also inflammation inducers. Rab27a governs the release of EPX and MBP from eosinophils, which leads to immune suppression or inflammation. Modulation of Rab27a can alleviate airway allergy responses by modulating eosinophil's immune suppressive functions, which has the translational potential for the management of eosinophil-related diseases.

Keywords: Airway; Allergy; Eosinophil; Immune regulation; Mucosal immunology.