Detection of H2O2 and catalase on a paper-based flow sensor constructed with borate cross-linked PVA hydrogel

Ruotong Li; Ranran Cheng; Jinpeng Liu; Yanhui Bi; Ping Song; Qiongzheng Hu; Li Yu

doi:10.1016/j.talanta.2024.126244

Detection of H₂O₂ and catalase on a paper-based flow sensor constructed with borate cross-linked PVA hydrogel

Talanta. 2024 May 15:276:126244. doi: 10.1016/j.talanta.2024.126244. Online ahead of print.

Authors

Ruotong Li¹, Ranran Cheng², Jinpeng Liu¹, Yanhui Bi², Ping Song³, Qiongzheng Hu⁴, Li Yu⁵

Affiliations

¹ Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China.
² Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, China.
³ School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
⁴ Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address: huqz@qlu.edu.cn.
⁵ Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China. Electronic address: ylmlt@sdu.edu.cn.

PMID: 38754185
DOI: 10.1016/j.talanta.2024.126244

Abstract

The detections of H₂O₂ and catalase play an important role in daily life. This study introduces a paper-based flow sensor that is specifically designed to detect H₂O₂ and catalase. The sensor utilizes a hydrogel composed of cross-linked 4-carboxyphenylboronic acid and polyvinyl alcohol. When H₂O₂ is in contact with the hydrogel, the B-C bonds of the hydrogel undergo a reactive process, causing decomposition of the hydrogel. The pH indicator strip enables the visual monitoring of the viscosity change that occurs during the gel-sol transition. The quantification of H₂O₂ is accomplished by assessing the proportion of water coverage on the pH indicator strip. The sensor shows a detection limit of 0.077 wt% and is applicable for the quantitative measurement of H₂O₂ in routinely used disinfectants. Furthermore, the presence of catalase is effectively identified and the detection of catalase in milk is successfully fulfilled. In summary, this work proposes a simple, user-friendly, label-free, and cost-effective method for constructing a paper-based flow sensor using borate cross-linked polyvinyl alcohol hydrogel, showing great potential for detecting H₂O₂ and catalase in various practical scenarios.

Keywords: Catalase; Distance; H(2)O(2); PVA hydrogel; Paper-based sensor; Viscosity.