The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis

Artif Cells Nanomed Biotechnol. 2024 Dec;52(1):300-308. doi: 10.1080/21691401.2024.2347377. Epub 2024 May 16.

Abstract

Lung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between A549 cells and Trichinella spiralis (T. spiralis). Our data showed that anti-Ts7TMR scFv could inhibit lung cancer growth in a dose-dependent manner, with a tumour inhibition rate of 59.1%. HE staining did not reveal any obvious tissue damage. Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer.

Keywords: A549 lung cancer; Trichinella spiralis; Ts7TMR; associated antigens; scFv.

MeSH terms

  • A549 Cells
  • Animals
  • Cell Proliferation* / drug effects
  • Humans
  • Lung Neoplasms* / drug therapy
  • Lung Neoplasms* / immunology
  • Lung Neoplasms* / metabolism
  • Lung Neoplasms* / pathology
  • Mice
  • Mice, Nude*
  • Neovascularization, Pathologic / immunology
  • Proliferating Cell Nuclear Antigen / immunology
  • Proliferating Cell Nuclear Antigen / metabolism
  • Single-Chain Antibodies* / immunology
  • Single-Chain Antibodies* / pharmacology
  • Trichinella spiralis* / immunology
  • Vascular Endothelial Growth Factor A / immunology
  • Vascular Endothelial Growth Factor A / metabolism
  • Xenograft Model Antitumor Assays