Electrochemical DNA-nano biosensor for the detection of Goserelin as anticancer drug using modified pencil graphite electrode

Layla Abd-Al-Sattar Sadiq Laylani; F Al-Dolaimy; Ali Altharawi; Ghasen M Sulaman; Mohammed Ahmed Mustafa; Adnan Taan Alkhafaji; Ali G Alkhatami

doi:10.3389/fonc.2024.1321557

Electrochemical DNA-nano biosensor for the detection of Goserelin as anticancer drug using modified pencil graphite electrode

Front Oncol. 2024 May 1:14:1321557. doi: 10.3389/fonc.2024.1321557. eCollection 2024.

Authors

Layla Abd-Al-Sattar Sadiq Laylani¹, F Al-Dolaimy², Ali Altharawi³, Ghasen M Sulaman⁴, Mohammed Ahmed Mustafa⁵, Adnan Taan Alkhafaji⁶, Ali G Alkhatami⁷

Affiliations

¹ Community Health Department, Kirkuk Technical Institute, Northern Technical University, Mosul, Iraq.
² Community Health Department, Al-Zahraa University for Women, Karbala, Iraq.
³ Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
⁴ Department of Medical Laboratories, Sawa University, Almuthana, Iraq.
⁵ Department of Medical Laboratory Technology, University of Imam Jaafar AL-Sadiq, Baghdad, Iraq.
⁶ Cardiology Department, College of Medicine, Al-Ayen University, Thi-Qar, Iraq.
⁷ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.

Abstract

Goserelin is an effective anticancer drug, but naturally causes several side effects. Hence the determination of this drug in biological samples, plays a key role in evaluating its effects and side effects. The current studies have concentrated on monitoring Goserelin using an easy and quick DNA biosensor for the first time. In this study, copper(II) oxide nanoparticles were created upon the surface of multiwalled carbon nanotubes (CuO/MWCNTs) as a conducting mediator. The modified pencil graphite electrode (ds-DNA/PA/CuO/MWCNTs/PGE) has been modified with the help of polyaniline (PA), ds-DNA, and CuO/MWCNTs nanocomposite. Additionally, the issue with the bio-electroanalytical guanine oxidation signal in relation to ds-DNA at the surface of PA/CuO/MWCNTs/PGE has been examined to determination Goserelin for the first time. It also, established a strong conductive condition to determination Goserelin in nanomolar concentration. Thus, Goserelin's determining, however, has a 0.21 nM detection limit and a 1.0 nM-110.0 µM linear dynamic range according to differential pulse voltammograms (DPV) of ds-DNA/PA/CuO/MWCNTs/PGE. Furthermore, the molecular docking investigation highlighted that Goserelin is able to bind ds-DNA preferentially and supported the findings of the experiments. The determining of Goserelin in real samples has been effectively accomplished in the last phase using ds-DNA/PA/CuO/MWCNTs/PGE.

Keywords: Goserelin; DNA biosensor; Voltammetry; carbon nanotubes; copper oxide.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for funding this work through small research program under grant number RGP.2-534-44.