An injectable bone paste of poly (lactic acid)/zinc-doped nano hydroxyapatite composite microspheres for skull repair

Xinrui Du; Xiaoting Yuan; Si Lin; Xinyu Tan; Yingchao Han

doi:10.1016/j.colsurfb.2024.113969

An injectable bone paste of poly (lactic acid)/zinc-doped nano hydroxyapatite composite microspheres for skull repair

Colloids Surf B Biointerfaces. 2024 May 13:239:113969. doi: 10.1016/j.colsurfb.2024.113969. Online ahead of print.

Authors

Xinrui Du¹, Xiaoting Yuan¹, Si Lin¹, Xinyu Tan¹, Yingchao Han²

Affiliations

¹ State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China.
² State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China. Electronic address: hanyingchao@whut.edu.cn.

PMID: 38749168
DOI: 10.1016/j.colsurfb.2024.113969

Abstract

In this study, poly (lactic acid)/zinc-doped nano hydroxyapatite (PLA/nano-ZnHA) composite microspheres were prepared and formed into injectable bone paste with sodium alginate (SA) and polyvinyl alcohol (PVA) for bone defect repair. The effect of component of bone paste on injectability and zinc doping content related biological properties were mainly discussed. An injectable bone paste of PLA/nano-ZnHA composite microspheres (CM) was formed in mass ratio of (2.5-25):(0.25-4): (0-2.5):(20-65) of CM, SA, PVA and water with the favorable injectability (average force:4.46±1.72 N). In vitro 5%-10% zinc doping content displayed significantly higher promotion on cell proliferation and osteogenic differentiation than 15%-20% zinc doping content. Furthermore, in vivo the significant promoting effect of 0-5% zinc doping in ZnHA on bone repair was observed. Although 5% zinc doping content did not show a significant enhancement in bone volume/tissue volume (BV/TV), it has the ability to improve the bone mineral density (BMD) in early stage of bone repair compared with the 0% zinc doping content. The PLA/nano-ZnHA composite microsphere injectable paste with convenient surgical operation and well filling ability has the potential to become a competitive tissue repair material.

Keywords: Bone paste; Composite microspheres; Osteogenic differentiation; Polylactic acid; Zinc-doped nano hydroxyapatite.